Second week
1. Lecture regarding plasmid.
2. Group members summarize the last group meeting:
• Concept: [金山文档] iGEM知识点整理2020dec27.docx
• Ways to operate website and applications.
Third week
1. Summarize previous meet.
2. The principle of primer design and related knowledge.
3. Primers design practice.
What we did:
1. Design primers
2. Summarized the procedure of primer design: [金山文档] 引物设计范例.docx
Wet lab
1. Read references
2. Design iGEM project
Model: https://2019.igem.org/Team:GENAS_China/Model
1. Write the modeling ideas in detailed.
2. Concluded pictures and text
3. Represent the complete solving process
Wiki：https://2019.igem.org/Team:TU_ Kaiserslautern
Remake the home page and learn simple website programming methods
HP
1. Draw up a sponsorship plan:
2. Design a human practice activity
Art
1. Design a team uniform:
2. Design a team logo
Wet lab
1. Read references
2. Design iGEM project
Model: https://2019.igem.org/Team:GENAS_China/Model
1. Write the modeling ideas in detailed.
2. Concluded pictures and text
3. Represent the complete solving process
Wiki: https://2019.igem.org/Team:TU_ Kaiserslautern
Remake the home page and learn simple website programming methods
HP
1. Draw up a sponsorship plan
2. Design a human practice activity
Art
1. Design a team uniform
2. Design a team logo
Wet lab
1. Read references
2. Design iGEM project
Model: https://2019.igem.org/Team:GENAS_China/Model
1. Rewrite the modeling ideas in detailed
2. Concluded pictures and text
3. Represent the complete solving process
Wiki: https://2019.igem.org/Team:TU_Kaiserslautern
Remake the home page and learn simple website programming methods
HP
1. Draw up a sponsorship plan
2. Design a human practice activity
Art
1. Design a team uniform
2. Design a team logo
Wet Lab:
PPT report documents: An engineered PET depolymerase to break down and recycle plastic bottles, Fungal Hydrophobin RolA Enhanced PETase Hydrolysis of Polyethylene Terephthalate, Surface displayexpression of Bacillus licheniformis lipase in Escherichia coli using
Dry Lab:
PPT presentation of modeling work
Art:
Uniform design:
Show the preliminary design uniform and explain
Logo design:
Show the preliminary design logo and explain
Wet Lab
Hydrophobins group：
PPT report documents
Hydrophobins Proteins that self assemble at interfaces
Bacillus Subtilis group:
PPT report document: An engineered PET depolymerase to break down and recycle plastic bottles Extend Fig. 4
Wet Lab
Hydrophobins group：
PPT report documents:
Two Forms and Two Faces, Multiple States and Multiple Uses: Properties and Applications of theSelf-Assembling Fungal Hydrophobins, Self-assembly ofhydrophobin proteins from the fungus Trichoderma reesei
Bacillus Subtilis group:
PPT report document：A dual tag system forfacilitated detection of surface expressed proteins in Escherichia coli, Comparison of the Organophosphorus Hydrolase Surface Display Using InaVN and Lpp-OmpA Systems in Escherichia coli, Decorating microbes surface display of proteins on Escherichia coli.》
Dry Lab:
https://2019.igem.org/Team:GENAS_China/Model
According to the model in the link, make a PPT for explanation
Art
Logo design:
Show and explain the modified Logo
Wet Lab
Hydrophobins group：
1. Write an email asking for LCC, ICCG and WCCG genes
2. Review the PCR principle, primer design principle and primer design method
3. Self-studyon principle of overlap PCR and design method of overlap PCR primer
Bacillus Subtilis group:
1. Write emails asking for bacillus
2. Read reference《热纤梭菌耐热酯酶的表达鉴定及枯草杆菌芽孢表面展示_张天喜》
Dry Lab:
https://2019.igem.org/Team:GENAS_China/Model
Learn how to make models
Wiki
Register personal account and team account
Art
Uniform design
Short-sleeved team uniform design
Wet Lab
Hydrophobins group：
1. Write an email asking for LCC, ICCG and WCCG genes
2. Review the PCR principle, primer design principle and primer design method
3. Self-studyon principle of overlap PCR and design method of overlap PCR primer
Bacillus Subtilis group:
1. Write emails asking for bacillus
2. Read reference《热纤梭菌耐热酯酶的表达鉴定及枯草杆菌芽孢表面展示_张天喜》
Dry Lab:
https://2019.igem.org/Team:GENAS_China/Model
Learn how to make models
Wiki
Register personal account and team account
Art
Uniform design
Short-sleeved team uniform design
Wet Lab
Hydrophobins group：
1. Quantity 50% glycerin
2. Ligation and Electro transformation for mLCC & mLCC-mHG & mLCC-BslA & pET28ainto E.coliDH5α
Bacillus Subtilis group:
1. Single digestion for PWB980 plasmid
2. Run the gel and gel recycle for CotC-mLCC, CotG-mLCC, CotX-mLCC
3. Design a seamless cloned primer for pHT43 carriers
4. Extraction of template plasmid CotB-mLCC and double digest verification success
Dry Lab:
Learn to model video
Wiki:
Team home page coding
Art
Uniform design
Complete the team uniform design
HP
Complete the plan of article publication on the public account
Wet Lab
Hydrophobins group：
1. PCR product mLCC & mLCC-mHGFI run the gel and gel recycle
2. Double digestion, run the gel and gel recycle for mLCC-BsLA & mLCC-mHGFI
3. Inoculation for mLCC-mHGFI
Bacillus Subtilis group:
1. PCR product CotC-mLCC & CotX-mLCC & CotG-mLCC & CotB-mLCC run the gel and gel recycle 2. PCR CotC-mLCC & CotX-mLCC & CotG-mLCC &CotB-mLCC
Dry Lab:
Learn to model video
Wiki:
Team home page coding
HP
Complete the outline of article writing for the public account
Wet Lab
Hydrophobins group：
1. Seamless cloning and transformation for mLCC
2. Extraction of plasmid mLCC-mHGI & mLCC-BsLA
3. Double digestion for mLCC-mHGI & mLCC-BsLA(verification success)
4. Run the gel and gel recycle for mLCC-mHGFI
Bacillus Subtilis group:
1. PCR run the gel and gel recycle for CotC-mLCC & CotX-mLCC & CotG-mLCC
2. PCR run the gel for CotB-mLCC
3. Seamlesscloning and Electro transformation for CotC-mLCC & CotX-mLCC & CotG-mLCC
Dry Lab
Learn to model video
Wiki
Complete the page framework coding
HP
Draft writing and translation of the public account
Wet Lab
Hydrophobins group：
1. Active cloning for mLCC2. Ligation and Electro transformation for mLCC-mHGI
Bacillus Subtilis group:
1. PCR run the gel for CotB-mLCC
2. Allocate 20 tablets
3. Singe-pick cloning for CotC-mLCC & CotX-mLCC & CotG-mLCC
Dry Lab
Learn to model video
Wiki
Coding the pages
HP
Draft writing and adding
Wet Lab
Hydrophobins group:
1. Transformation for mLCC & mLCC-mHG & mLCC-BslA & mLCC-mHFBI
Bacillus Subtilis group:
1. Extraction of plasmid CotB-mLCC & CotC-mLCC & CotX-mLCC & CotG-mLCC
2. PCR run the gel for CotB-mLCC
3. Double digestion for CotC-mLCC & CotX-mLCC & CotG-mLCC
4. Run the gel and gel recycle for CotC-mLCC & CotX-mLCC & CotG-mLCC
Dry Lab
Learn to model video
Wiki
1. Coding the pages
2. Webpage content writing (Notebook)
HP
Typesetting the article for public account
Art
Draw the home page picture
Wet Lab
Hydrophobins group:
1. Ligation for mLCC with PET28a and Electro transformation
2. Singe-pick cloning and shaking tablet for mLCC & mLCC-mHG & mLCC-BslA & mLCC-mHFBI
3. Extraction of plasmid mLCC-mHFBI and double digest verification
Bacillus Subtilis group:
1. Run the gel for digestion CotC-mLCC & CotX-mLCC & CotG-mLCC
2. Run the gel for CotB-mLCC
3. Transformation for pHT43
Dry Lab
Learn to model video
Wiki
1. Coding the pages
2. Webpage content writing (Notebook)
Art
Draw the home page picture
Wet Lab
Hydrophobins group:
1. Glycerol-preserving bacteria
2. Allocatealbumengel
3. IPTG induction for mLCC & mLCC-mHG & mLCC-BslA & mLCC-mHFBI
4. Prepare the pre-induction and post-induction bacterial solutions for mLCC & mLCC-mHG & mLCC-BslA & mLCC-mHFBI
Bacillus Subtilis group:
1.Single-pick cloning for pHT43
Dry Lab
Learn to model video
Wiki
Coding the pages
HP
Discuss the online meet up content
Art
Draw the home page picture
Wet Lab
Hydrophobins group:
1. Run the albumen gel for mLCC & mLCC-mHG & mLCC-BslA & mLCC-mHFBI
2. Single-pick cloning for mLCC
Bacillus Subtilis group:
1. Extraction of plasmid CotB-mLCC & CotC-mLCC & CotX-mLCC & CotG-mLCC
27℃ digestion for CotB-mLCC & CotC-mLCC& CotX-mLCC & CotG-mLCC
3. Run the gel and gel recycle for CotB-mLCC& CotC-mLCC & CotX-mLCC & CotG-mLCC
Dry Lab
Learn to model video
Wiki
Coding the pages
Art
Draw the home page picture
Wet Lab
Hydrophobins group:
1. Bacterial culture for mLCC & mLCC-mHG & mLCC-BslA & mLCC-mHFBI
2. Double digestion for mLCC
Bacillus Subtilis group:
1. Ligation and transformation for pHT43 & CotC-mLCC & CotX-mLCC & CotG-mLCC
Dry Lab
Preliminary discussion modeling
Wiki
1. Coding the pages
2. Webpage content writing (Members)
Art
Draw the home page picture
Wet Lab
1. Prep LB liquid Medium
2. Sterilization
3. Configuring protein glue (for future use)
4. Material Preparation
Wet Lab
1. Follow-ups work
2. Protein Induction
3. Osteoclasts
HP
1. Team Meet-up (Offline meeting between the two teams)
Wiki
1. Coding
2. Web page content writing
Wet Lab
1. Protein Purification
2. Relative molecular weight of protein
Wiki
1. Coding
2. Web page content writing
Art
1. Sketch Map
Wet Lab
1. Protein centrifugation
2. Protein electrophoresis
Wiki
1. Coding
2. Web page content writing
Art
1. Sketch Map for Wiki
Model
1. Team Meet-up (Offline meeting between the two teams)
HP:
1. Web page content Writing
Wiki:
1. Coding
2. Web page content writing
Art:
1. Sketch Map for Wiki
Model
1. Data collecting
Wet Lab:
1. Protein electrophoresis
2. Run Errands
HP:
1. Web page content Writing
Wiki:
1. Coding
2. Web page content writing
3. Learning Pymol
Art:
1. Multiple Sketch Map for Wiki
Model
1. Report Progress
HP:
1. Web page content writing
Wiki:
1. Coding
2. Web page content writing
3. Learning Pymol
Model
1. Web page content writing
Wet Lab:
1. Doing experimental close-out work
HP:
1. Web page content Writing
2. Holding Integrated HP activity
Wiki:
1. Coding
2. Web page content writing
Art:
1. Multiple Sketch Map for Wiki
Model
1. Learning MD
HP:
1. Web page content Writing
2. Participating in the Plasticase online roundtable
Wiki:
1. Coding
2. Web page content writing
3. Register parts
Art:
1. Illustration of the web pages
Model
1. Learning MD
2. Web page content writing
Record the presentation video
1. Brief introduction (regarding: the competition.)
2. Basic biology concept went through (gene, DNA, protein).
3. Download the basic applications on the computer.
4. Get familiar with common website.
What we've done:
1. Learning Video content.
2. Download apps.